High throughput process development for the purification of rapeseed proteins napin and cruciferin by ion exchange chromatography

Proteins derived from plant resources such as oilseed meals, canola and sunflower, are considered a viable alternative to animal proteins for food consumption. This work presents rational methodology, using high throughput experimentation (HTE), the separation of cruciferin napin, two major meal, by chromatography. Eight different mixed mode ion exchange resins were evaluated at conditions with aim capturing napin identifying adsorption/desorption behavior, ease desorption selectivity. POROS 50 HS resulted most promising resin. The obtained equilibrium adsorption data was used in mechanistic chromatography model compared experimental results showing very good agreement. identify column operating parameters that lead >98% yield purity both proteins. Subsequently conceptual downstream processing proposed.